BIO230H1 Lecture Notes - Lecture 5: Guanine, Dna Microarray, Drug Discovery

• School: UTSG
• Department: Biology
• Course: BIO230H1
• Professor: Darrell Desveaux Tony Harris

Document Summary

Thursday, september 21st, 2017 1pm-2pm (alberts et al. Lecture 5 outline: eukaryotic transcriptional repression (cont"d , post-transcriptional regulation, rna processing, rna nuclear export. The fingerprint of gene expression (transcriptome) can be used for drug development. Transcriptome is entire set of transcripts possessed by a cell type or tissue at a given time: entire set of rna molecules, different cell types express different genes and at different levels. Transcriptome provides signature of cell state and also responds to extracellular stimuli which could be a drug treatment. Possible because we can screen through thousands of chemicals with drug-like properties and are very structurally diverse so you can look for the phenotype you"re interested in. Transcriptome analyses: transcriptome provides a signature of cell state, blue: low expression, red: high expression. Ic50: concentration of compound required to inhibit cell proliferation by 50% Show subset of genes that show differences between healthy and diseased cells. We want to stop proliferation and kill them eventually.