BIO230H1 Lecture Notes - Lecture 3: Synthetic Biology, Prophase, Cyclic Adenosine Monophosphate
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BIO230H1 Full Course Notes
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Viral phage uses to control its lifecycle. Also genetic circuits that can be formed between different parts of gene. Gene regulatory proteins bind to regulatory elements of dna expression profiles. How genes are regulated by gene regulatory proteins: 2 overall types of gene regulatory proteins influenced by small molecules. [1] turn on (activators/positive regulation) (a) activator bound only when small molecule bound to it. Needs to be bound to camp = small molecule (b) activator is removed when small molecule bound to it camp produced when glucose goes down. When small molecule is bound, activator is not bound. [2] turn off (repressors/negative regulation) (a) repressor normally bound to specific site called operator. Small molecule binds to repressor and decreases its dna binding affinity. Lac repressor falls off allowing for expression of lac operon if no glucose in media (b) repressor has higher affinity for dna when bound to small molecule. Uses protein sequences specialized for binding dna:
