BIO130H1 Lecture Notes - Lecture 6: Nuclear Localization Sequence, Hydrolysis, Neutrophil

31 views8 pages

ultramarineyellowjacket745

29 Mar 2017

School

Department

Course

Professor

For unlimited access to Class Notes, a Class+ subscription is required.

ash-mr18 and 40043 others unlocked

BIO130H1 Full Course Notes

Verified Note

31 documents

Document Summary

Transmembrane protein from translation to the plasma membrane (remains transmembrane) Cytosol -> er: translocation into the er membrane co-translational translocation (while you are translating it, you are trans locating it, requires a signal sequence. Ribosome binds to the mrna (in the cytosol) -> ribosome starts making the protein -> make the internal signal sequence which wants to go to the er. The srp and srp receptor are still involved in this process. Hydrophobic transmembrane segment this is the internal signal sequence -> from here, it is either step 1 followed by 2a, 3a, 4a or 1 followed by 2 and 3. Protein at the trans locator (charged) -> it will detect and look at the amino acids next to the internal signal sequence and essentially attract the opposite charge. Second case scenario: n terminal side is more while the cytosol side is more + -> n terminus stays in the er lumen and.

Related Questions

please help me answer theses questions correctly

1/ Which of the following are required for one-pass transmembrane proteins?

I. N-terminal signal sequence

II. Binding of SRP to the SRP receptor

III. Presence of stop-transfer sequence

IV. Presence of start-transfer sequence

a) I and III

b) I, II, and III

c) II, III, and IV

d) I, II, III, and IV

___________________

2/ Which of the following statements about the signal-recognition particle (SRP) is FALSE?

a) The SRP binds to the ER signaling sequence after the entire protein has been translated by the ribosome.

b) The SRP cycles between the ER membrane and the cytosol.

c) The SRP is required for peptides destined for either the ER membrane or ER lumen.

d) The SRP recognizes the signal sequence at the N-terminus of the newly forming polypeptide.

______________________

3) t-SNARES are _____.

a) found on the cytosolic side of the vesicle membrane.

b) necessary for recognition and transport of proteins into the nucleus.

c) usually located on the target membrane.

d) necessary for vesicle formation.

ceruleanchimpanzee878

1. Which cytosolic factor is most likely to associate with the signal sequence of a protein that is targeted co-translationally to the ER?

A chaperone of the Hsp70 family

The translocation channel

The ribosome

Signal recognition particle (SRP)

Signal peptidase

2. What is the most likely fate of a protein with an N-terminal hydrophobic sorting signal and an additional internal hydrophobic domain of 22 amino acids?

The protein stays in the cytosol

The protein is transported to mitochondria

Because the protein has an N-terminal sorting signal, the protein is translocated all the way into the ER lumen

The hydrophobic domain is recognized as a transmembrane domain once it is in the translocation channel and released sideways into the membrane

The hydrophobic domain is cleaved off by signal peptidase

3. Which of the following statements best describes N-glycosylation? N-glycosylation refers to........

the addition of a pre-formed sugar tree to a serine or threonine present in the cytosolic domain of a protein

the addition of a pre-formed sugar tree to a phospholipid to make a glycolipid

the addition of individual sugars to selected amino acids on a protein

the addition of a pre-formed sugar tree to an asparagine residue found within the consensus sequence Asn-X-Ser/Thr in an ER lumenal domain of a protein

the addition of a pre-formed sugar tree to an asparagine residue found within the consensus sequence Asn-X-Ser/Thr in a cytosolic domain of a protein

4. What is the most likely fate of a protein in which a mutation has led to misfolding in the ER?

It is sequestered by the proteasome in the cytosol

It is first tagged with ubiquitin in the ER and then degraded by the proteasome in the cytosol

It is first moved out of the ER, tagged with ubiquitin in the cytosol and then degraded by the proteasome in the cytosol

It associates with many chaperones in the ER, which guarantee its correct folding

Its misfolded state is recognized by chaperones in the ER, which will induce the protein's degradation by the proteasome in the ER.

raspberrybeaver682

#1 Which of the following must occur when a position on the ER isnot available for a ribosome, which is translating a secretoryprotein

A The ribosome will temporarily dock on the smooth ER
B The ribosome will disassemble until there is an availableposition
C The ribosime will undergo translational arrest
D The ribosome will complete the synthesis of the protein and dropit into the cytosol as a domesticated protein

#2 N-glycosylation occurs in which organelle?

A RER
B SER
C Golgi Apparatus
D All of the above

#3 A protein with two stop-transfer sequences along with aLys-Lys-Met-Ser sequence will end up where in the cell?

A Plasma Membrane
B ER Membrane
C The ER lumen
D The lysosome

#4 Which does NOT happen during co-translation of secretoryproteins?

A Cleaving of protein
B Glycosylation of proteins
C Trimming of protein
D Protein folding

#5 Which is the CORRECT order the translational complexforms?

A mRNA---> large ribosome ---> small ribosome --->tRNA
B Large ribosome ---> mRNA ---> small ribosome --->tRNA
C Small ribosome ---> large ribosome ---> mRNA --->tRNA
D mRNA ---> small ribosome ---> large ribosome --->tRNA

#6 Which component of the cytoskeleton consist of rope-likefibrills that provides the cell mechanical strength when subjectedto physical stress, including neurons, muscle cells, and theepithelial cells that line the body's cavities?

A Microtubules
B Microfiliaments
C Intermediate Filiaments
D Lamins

#7 What type of glycosylation occurs in the Golgi?

A Glycosylation of Asparagine
B Glycosylation of Serine
C Glycosylationof Asp and Ser
D Glycosylation of Nucleic Acids

#8 Ebony was examining a cell's organelle which was almost as largeas the cell. She discovered that the pH was 4.6. Which of thefollowing organelles was she studying?

A Nucleus
B Endosome
C Vacuole
D Lysosome

#9 Which is NOT a function of the smooth ER?

A Synthesis of carcinogens
B Synthesis of lipids, carbohydrates, and nucleic acids
C Detoxification
D Release of calcium and glucose

#10 Which signal sequence is NOT part of the final protein?

A Glycosylation
B Stop-transfer
C Leader sequence
D ER retention

#12 Detoxification of charred foods oftentimes form carcinogensfrom:

A acid hydrolases
B benoapadines
C benzopyrenes
D free radicals

BIO130H1 Lecture Notes - Lecture 6: Nuclear Localization Sequence, Hydrolysis, Neutrophil

BIO130H1 Full Course Notes

Document Summary

Get access

Related textbook solutions

Molecular Cell Biology

Biology: Science for Life with Physiology

Biology

Concepts of Biology

Essentials of Biology

Related Documents

BIO130H1 Lecture Notes - Lecture 18: Golgi Apparatus, Endoplasmic Reticulum, Signal Peptidase

BIO130H1 Lecture Notes - Lecture 15: Signal Recognition Particle, Transmembrane Protein, Cytosol

BIO130H1 Lecture Notes - Lecture 18: Golgi Apparatus, Translocator Protein, Transmembrane Protein

Related Questions