CHM362H5 Lecture Notes - Lecture 18: Endergonic Reaction, Glycolysis, Red Blood Cell

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2 Jan 2016
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Reaction is slightly endergonic: 1,3-bisphosphoglycerate is a high-energy phosphate compound. Glycolysis: reaction 7: break-even reaction, phosphoglycerate kinase transfers pi from 1,3-bpg to adp to form atp, because phase 1 yields 2 molecules of 1,3-bpg, 2 atp molecules formed in this step. Offset 2 atp molecules used in phase 1. Glycolysis: reaction 7, cont"d: can view reactions 6 and 7 as coupled reactions. Overall g "rxn6+7 for coupled reaction also negative. Used a pi from substrate 1,3-bpg to phosphorylate adp to make atp: although phosphoglycerate kinase reaction has large negative g " (=-18. 9 kj/mol), Relatively high [atp] and [3-phosphoglycerate] in blood cells; q > keq". In blood, 3-phosphoglycerate is phosphorylated from atp to make 1,3-bpg. Large negative g " used to bring previous 3 reactions closer to equilibrium. Another his in enzyme: catalytic his must be phosphorylated before catalysis, 2,3-bisphosphoglycerate provides the phosphate. Glycolysis: reaction 9: generation of high energy phosphate compound! Needed to generate atp in final step of glycolysis.

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