BIO206H5 Lecture Notes - Lecture 5: Bamhi, Methyltransferase, Sticky And Blunt Ends
Document Summary
Nucleases are enzymes that cleave phosphodiester bonds between nucleotides of nucleic acids cut nucleic acids from the 5" or 3" nucleases: Endo degrades nucleases: cut nucleic acids internally . What are they? a subset of endonuclease enzymes that cut dsdna at specific dna sequences identified by the enzyme itself endonucleolytic cleavage of dna to give specific double stranded fragments with terminal 5"-phosphates smart dna scissors. Re"s catalyzes endonucleolytic cleavage of dna to give specific double-stranded fragments with terminal 5"- phosphates cuts the dna at the aatt area as there are only 2 h-bonds between the base pairs. Ecori methylase: regulates the re, addes methyl group to base pair so then restriction enzyme ecor1 can not bind and cut that area. 4 classes of restriction endonucleases: type i: require atp, sam and cut away from recognition site. Type ii: most useful for molecular biology, requires mg2+, but not. Atp or sam; cut site is at the recognition site.
