BIO206H5 Lecture Notes - Lecture 9: Rna Polymerase Ii, Rna Polymerase Iii, Rna Polymerase I

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1 Oct 2016

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Larger genome more genes to recognize and transcribe. Dna organization: tightly condensed chromatin with many proteins. Rna molecule is segregated from the region where the mrna is translated into a protein. Organization of the dna within the chromatin is more complicated and much more difficult to unwind. No really specific transcription termination site like that of a prokaryotic cell. Introns are intervening sequences that will be removed. 3 stages of transcription: initiation: binding of rna polymerase to a promoter sequence, elongation: sequential addition of ribonucleotides as prescribed by the dna template strand. 3)termination: dissociation of rna polymerase release of primary transcript from template. 3 primary processing events can occur: 5" end and 3" end modified, introns removed, exons spliced together. Processing of rna occur only in eukaryotic cells; for prok, rna does not necessarily need to be completed before protein translation begins; co-trancription and translation.

Related Questions

Eukaryotic transcription normally takes place on a chromatin template at nuclease-accessible sites.(True/False)

*After promoter clearance by RNA polymerase II:

A) all associated factors remain bound to the polymerase for elongation.

B) a subset of factors remain at the promoter region.

*Which of the following statements is FALSE?

A) RNA polymerase II is responsible for the transcription of genes encoding protein-coding sequences only.

B) Many promoters recognized by RNA polymerase II contain regulatory elements several kbp upstream of the initiation site.

C) TFIIB has a role in converting a closed-promoter into an open-promoter complex.

D) The C-terminal domain of the largest subunit of RNA pol II undergoes extensive phosphorylation prior to transcription elongation.

E) A multiprotein complex is involved in communication between upstream control elements and proteins bound at the promoter.

C) no further elongation factors are acquired by the polymerase.

D) no further chromatin remodeling is required.

E) transcription continues until the polymerase reaches the poly (A) tail

*In eukaryotes RNA polymerase I transcribes all of the ribosomal RNA components.(TRUE/FALSE)

1 DNA sequences found in introns provide [A] amino acid sequence information.

[B] regulatory information.
[C] no known useful information.

[D] structure for the gene.
[E] alternative DNA splicing possibilities.

3. The TATA box is a(n)
[A] sequence close to the promoter region of many genes.
[B] square-shaped sequence.
[C] enhancer consensus sequence.
[D] activator sequence necessary for proper translation.
[E] None of the above

5. Regarding genes and gene expression, which of the following is not a difference between prokaryotes and eukaryotes
[A] Eukaryotic DNA does not contain a TATA box close to the initiation site as does prokaryotic DNA.

[B] In eukaryotes, RNA polymerase requires

'transcription factors'or initiation.

[C] Prokaryotic genes are often grouped together in

operons.

[D] In eukaryotes, the regulation of several genes at once requires common control elements in each of the genes.
[E] There is a diversity of eukaryotic polymerases that is reflected in the diversity of eukaryotic promoters.

10) In eukaryotic cells, a repressor

[A] is made of DNA.
[B] binds to the enhancer region to block transcription. [

C] is located both upstream and downstream from the promoter.
[D] binds to the operator to block RNA polymerase.

[E] binds to a silencer to reduce transcription rates.

Coordinated regulation of genes in eukaryotic cells [A] is the result of positioning the same regulatory sequence in front of each gene.
[B] results from all of those genes being under the control of one promoter.

[C] occurs because related genes all have the same

operons.
[D] occurs because enhancers cause DNA to bend.

22. What is probably the single most important distinction between bacteria and eukaryotes?

a. transcription

b. the separation of the cell's genetic material from the surrounding cytoplasm

c. the existence of ribosomes

d. RNA processing in bacteria

e. antibiotics

23. Which one does NOT characterize an open reading frame (ORF)

a. Uses six reading frames during translation

b. Contains a start codon

c. Contains a stop codon

d. Its length is in multiples of 3 nucleotides

e. It should have the number of codons for a protein or on average ~250 codons in length.

24. Nested intronic genes lie within the non-coding intronic region of a larger gene, and occur in the human genome.

a. True

b. False

25. How would you characterize this statement: EF-Tu will hydrolyze GTP only if the codon:anticodon & tRNA:amino acid matchings are correct

a. Transcription; initiation steps

b. Transcription; termination steps

c. Translation: initiation steps

d. Tanslation: elongation steps

e. Spliceosome

26. Regarding the transcription unit architecture of the Escherichia coli genome, which one is FALSE?

a. Each of the 4,661 transcription units comprised an average of 1.1 modular units.

b. 3,010 transcription units (~65%) are monocistronic.

c. 1,652 transcription units (35%) contain more than one ORF (polycistronic)

d. The largest transcription unit is equivalent to 16 ORFs with numerous introns.

e. 398 transcription units (~9%) are nested within each other in opposite transcriptional orientation

27. Regarding RNA interference (RNAi), which one is FALSE?

a. It is a common and conserved mechanism in eukaryotic cells.

b. Originally discovered in bacteria and plant viruses.

c. A form of primitive immunity to protect the genome.

d. Pre microRNA (miRNA) genes have been found in introns.

e. Recent research shows that exogenous plant miRNAs in food can regulate the expression of target genes in mammals.

28. Aminoacyl-tRNA synthetases

a. Recognize the 3âUTR

b. The genes for these enzymes fall into the family of inducible genes

c. The enzymes are imported into the nucleus

d. Covalently attaches an amino acid to the appropriate tRNA. This takes place in the cytoplasm.

e. Forms the peptide bond during protein synthesis

29. What is the process by which complementary RNAs are made from a DNA template?

a. translation

b. transcription

c. protein synthesis

d. transduction

e. transliteration

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BIO206H5 Lecture Notes - Lecture 9: Rna Polymerase Ii, Rna Polymerase Iii, Rna Polymerase I

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