BIO200H5 Lecture Notes - Lecture 7: Surface Plasmon Resonance, Surface Plasmon, Cytokine Receptor
Document Summary
Its end goal is to measure the effects of the drug on the body. Fraction of free receptor decreases with increasing drug (or ligand), until saturation occurs (bmax) Kd = concentration of drug (mol/l) when receptors are 50% bound. It is an alternate way of calculating kd. Light is passed through a prism that hits gold and bounces off at various directions hitting and missing targets. When the drug binds to the receptor, the light changes its angle. You can find the increase signal/time(kon) and the decrease signal over time (koff) in order to find kd, since kd= koff/kon. It can be measured without kd, and can be performed in vitro. Effect of the drug increases at the concentration of the drug increases. Gives information about [drug] that will elicit expected response. Need to confirm ic/ec50 in several in vitro and/or ex vivo experiments before moving into animals. Helps determine what [drug] to start with in animals.
