BIO 3170 Lecture Notes - Lecture 14: Dna-Binding Domain, Beta Sheet, Zipper
11 Apr 2018
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Document Summary
Transcriptional: controls the frequency of transcription initiation of a gene mrna maturation: quality control, splicing mrna transport and localization: distribution to cellular compartments. Translation: control at the level of mrna decoding mrna stability: transcript half life. Rdbps can recognize their target with a high degree of speci city. Dna binding domain: responsible for binding to the regulatory dna sequence: interaction depends on sequence and secondary structures, possible motifs: beta sheets, zinc ngers, alpha helices. Protein-protein interaction domains: in uence the transcriptional activity of the target gene through association with other rdbps or with components of the transcriptional machinery: dimerization domain, possible motifs: alpha helices, lucine zipper. Helix-turn-helix (hth) motif: 2 alpha helices connected by a short stretch of aa"s. Present in rdbps of both prokaryotes and eukaryotes. Contains a c-terminal dna-binding domain that takes the form of an alpha-helix: rich in arginines and lysines. Interacts primarily with the major groove through the ctd.
