BIO 1140 Lecture Notes - Lecture 6: Atp Synthase, Thylakoid, Adenosine Triphosphate

• School: University of Ottawa
• Department: Biology
• Course: BIO 1140
• Professor: Caroline Petit- Turcotte

Document Summary

Mitochondria are essential for many physiological functions including: The mitochondria is composed of two membranes (outer and inner) which define two compart- ments, the inter membrane space (found between the inner and outer layers) and the matrix (in- terior aqueous environment). It"s cristae (interfolds) increase the surface area within the matrix, allowing greater atp production. To study this organelle of a cell, we use a number of methods such as different gradients, buf- fers and speeds to isolate different components of the cell. This can also be done to break down the organelles into different areas to see which proteins are present, how they interact and whether or not they are imbedded in the membrane. Centrifugation, sub cellular fractionation, differential centrifugation, density gradient centrifugation and equilibrium density centrifugation are all methods that can be used. Nucleotide adenosine triphosphate is the form of energy that is supplied to drive endergonic re- actions within the cell.