BIOL239 Lecture Notes - Lecture 2: Dihybrid Cross, Lentil, Epistasis

32 views3 pages

raspberryduck719

22 Mar 2016

School

University of Waterloo

Department

Biology

Course

BIOL239

Professor

Christine Dupont

For unlimited access to Class Notes, a Class+ subscription is required.

Document Summary

The genotype and phenotype raios are both 1:2:1. Tells us that the gene that we are dealing with does not code for an enzyme, the pigment is just a protein product. We see a blending of colours in the ofspring: R1 produces the wild type red pigment. R2 produces white (a lack of red pigment) When red combines with white, they produce a pink pigment in the ofspring, because there isn"t suicient red pigment from just one red parent to make a red ofspring. If you start out with two grey cows and get a black cow ofspring: Not true breeding parents, because then you would keep geing grey ofspring. One hypothesis is that the colour is incomplete dominance (the black cow is homozygous for the black color allele) You make up a gene and its various alleles, cb and cw grey is heterozygous, so cbcw.

Related Questions

Problem A. You cross two homozygous parents: RRYY (round yellow) and rryy (wrinkled green) to produce an F1 generation. Round seeds are dominant, yellow seeds are dominant. You cross two F1s to produce the F2 generation. In the F2 generation, you find an individual with round yellow seeds. You do a testcross to find out the genotype of this âmysteryâ dominant/dominant phenotype. In the offspring of the cross, you find 1?2 have round yellow seeds and 1?2 have wrinkled yellow seeds.

1. Based on these data what was the genotype of the mystery F2?

Problem B. You carry out a dihybrid cross between a red-flowered rose with long pollen grains (A1A1 LL) and a white-flowered rose with round pollen (A2A2 l l). The flower color gene (A) shows incomplete dominance, and long pollen is completely dominant to round pollen. The genes for these traits are on different chromosomes so assort independently

2. What would be the phenotypes of the F1 generation?

3. What fraction of the F2 generation is expected to have red flowers and long pollen? (As always when traits are independent, what rule can you apply to save time?)

Problem D. In the example of epistasis presented in class, the agouti allele in mice (A) codes for striped hairs and is dominant to the allele for all black hairs (a). A loss-of-function allele for the pigment gene (b) produces no pigment and is recessive to the normal, dominant allele (B). The recessive allele at b masks the effect of the A gene. A dihybrid cross of AABB and aabb is carried out.

5. Name all of the genotypes in the F2 generation that can produce an albino (no pigment) phenotype.

Problem E. A cross is made between homozygous fruit flies that differ for eye color (the st gene) and

body color (the e gene). The F1 double heterozygote is shown below using chromosome notation.

st+ e

--------------------------------------

st e+

st = the recessive allele for scarlet eyes; st+ = the dominant wildtype allele for red eyes e = the recessive allele for ebony body; e+ is the dominant wildtype allele for gray body

6. Are the chromosomes shown in the genotype above in cis or trans configuration?

7. Based on this notation, what were the genotypes and phenotypes of the two parents of the F1? (Think carefully here...)

8. With what phenotype would you cross this F1 to measure recombination frequency?

cyansnail823

Q 1 â Manx cats are produced when a dominant allele is inherited for the Manx gene (M). Inheritance of two dominant alleles, however, results in death of the fetus. Coat color in cats is determined by two alleles with incomplete dominance. BB produces black colored cats, Bb produces grey cats, and bb produces white cats. A grey Manx cat is mated with a white Manx. What proportion of the living offspring will be white Manx kittens? (3 points)

Q 2 â In goats, a beard is produced by an autosomal allele that is dominant in males and recessive in females. Weâll use the symbol B^b for the beard allele and B+ for the beardless allele. Another independently assorting autosomal allele that produces a black coat (W) is dominant over the allele for white coat (w). Give the phenotypes and their proportions expected for the following cross: B⁺B⁺Ww male Ã B^bB^bWw female (2 points)

Q 3 â E. W. Lindstrom crossed two corn plants with green seedlings and obtained the following progeny: 3583 green seedlings, 853 virescent-white seedlings, and 260 yellow seedlings.

Give the genotypes for the green, virescent-white, and yellow seedlings

Explain how color is determined in these seedlings (which genotype gives which phenotype). (3 points)

Q 4 â You are studying a gene that controls ossicone (horn) length in giraffes. The wild-type long-ossicone allele (L) is dominant to the mutant short-ossicone (l) allele. However the L allele is only 60% penetrant in both homozygotes and heterozygotes. In a population of 212 giraffes in an African nature preserve, 80 giraffes are either homozygous or heterozygous for the long ossicone allele (L). What proportion of offspring would you expect to exhibit the long ossicone phenotype? (2 points)

harlequinserval672

11. You suspect that two genes interact with one another to determine the product of a pathway you are studying. You cross two pure breeding plantsâone with white flowers and one with pinkâto make F1 individuals that have blue flowers. You cross the F1 generation and observe the following numbers of individuals with different phenotypes: Number of individuals Phenotype 91 Blue 30 Pink 39 White Which pathway best explains these results? (Note that arrows indicate enzymatic reactions carried out by products of genes 1 and 2. Assume that buildup of metabolites will determine flower color.)

Question 12 and 13: Consider the biochemical pathway presented below. This pathway determines pigment production in pea flowers. (Note: gene products are required for each reaction to proceed.)

12. Do you expect that genes A and B will interact with one another?

A. Yes B. No C. Insufficient information to determine

13. What phenotype ratio would you expect in offspring produced by crossing AaBb parents?

A. 9:3:3:1 (blue : green : yellow : white) B. 9:3:4 (blue : green : white) C. 9:7 (blue : white) D. 15:1 (blue : white)

14. How can haplosufficiency explain dominance of an allele that codes for a functional protein over a null (loss of function) allele?

A) a single functional copy of the gene produces sufficient gene product for normal biological function B) a single functional copy of the gene produces insufficient gene product for normal biological function C) the null allele produces a product that interferes with proper function of the normal allele D) the null allele produces a product that does not interfere with proper function of the normal allele E) the null allele rapidly mutates to reacquire normal functionality

15. In a plant species with genes A and B, you have isolated recessive mutant alleles at each locus, which you call a and b. You would like to know whether these two genes are linked. To test this hypothesis, you obtain an individual that is heterozygous at both genes (AB/ab) and perform a test cross. Which results would be most consistent with these loci being tightly linked? (Results are counts of the numbers of offspring from the testcross.)

A. Ab/ab 390 aB/ab 410 AB/ab 350 ab/ab 420

B. Ab/ab 80 aB/ab 73 AB/ab 760 ab/ab 754

C. Ab/ab 700 aB/ab 695 AB/ab 56 ab/ab 59

D. Ab/ab 400 aB/ab 65 AB/ab 50 ab/ab 659

goldclam880

BIOL239 Lecture Notes - Lecture 2: Dihybrid Cross, Lentil, Epistasis

Document Summary

Get access

Related Documents

BIOL239 Chapter Notes - Chapter 2: Pigment, A Aa, Halothane

BIOL239 Lecture Notes - Summer Squash, Retina, Statistical Hypothesis Testing

BIOL239 Lecture Notes - Lecture 2: Lethal Allele, Epistasis, Penetrance

Related Questions