BIOC 4580 Lecture Notes - Lecture 7: Leishmania, Transmission Electron Microscopy, Membrane Protein
3 Feb 2017
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Document Summary
Membrane proteins some varieties of 3d structures. Membrane proteins are classified partly by the way in which they are extracted from a membrane, which reflects how strongly they are associated with it. Integral membrane proteins are further sub-classified by the number of times they span the bilayer. Such proteins are either largely a-helical or b-barrels & there are some general rules regarding their amino acid positioning. Human erythrocyte glycophorin, bacteriorhodopsin, and the photosynthetic reaction centre have already been or will be exemplified. All membrane proteins have annunalr or boundary lipids. Some membrane proteins are anchored to the membrane at least in part by covalently- attached lipids or fatty-acyl chains. Structure, topology, carbohydrate modification were all determined biochemically. 1st membrane protein with solved structure, initially by electron crystallography, later verified by x-ray crystallography. Bacteriorhodopsin is found in cytoplasmic membrane of halophilic (salt-loving) purple photosynthetic bacterium halobacterium salinarum. Does not rely on oxygen as much as other bacteria.
