GENE20001 Lecture Notes - Lecture 14: Cyclic Permutation, Concatemer, Enterobacteria Phage T4
Document Summary
* li(cid:374)ear dna is i(cid:374)serted i(cid:374)to the host (cid:272)ell & the dna (cid:272)ir(cid:272)ularises. * the progeny phage have linear dna in their head. * at the ends of the ge(cid:374)o(cid:373)e there are si(cid:374)gle stra(cid:374)ded parts called cos ends. They are sticky ends (they are complimentary sequences) * the two ends come together and they base pair to form a circle (cid:271)ut it is(cid:374)"t a (cid:272)o(cid:448)ale(cid:374)tl(cid:455) (cid:272)losed circle (cid:271)e(cid:272)ause there is(cid:374)"t a phospodiester bond between g&a or g&g. * there is an origin of replication (specific dna sequence to which dna replication proteins bind) * these dna replication proteins initiate dna replication. * this dna replication is semi-conservation (one old strand and one new strand) * these proteins melt/unhybridize at the origin which leaves single stranded exposed bases upon (cid:449)hi(cid:272)h dna (cid:272)a(cid:374) (cid:271)e s(cid:455)(cid:374)thesised (cid:271)(cid:455) ligati(cid:374)g a pri(cid:373)er a(cid:374)d the(cid:374) e(cid:454)te(cid:374)di(cid:374)g the (cid:1007)" e(cid:374)d of the pri(cid:373)er. * there are two replication forks going in opposite directions.
