SLE206 Lecture Notes - Lecture 2: Isoelectric Point, Gel Electrophoresis, Isoelectric Focusing

• School: Deakin University
• Department: Science, Engineering and Built Environment
• Course: SLE206
• Professor: Jessica Gibbons

Document Summary

Proteins and cell evolution - class 2b learning objectives. To appreciate the structural and functional diversity of proteins. Structurally proteins are very varied in size and shape. Transport: carry small molecules or ions: motor: generate movement in cells and tissues. Signal: carry extracellular signals from cell to cell. Receptor: detect signal and transmit them to response machinery. Gene regulator: bind to dna to switch genes on or off. A chain of amino acids is called a polypeptide. Each peptide has an amino terminal (n) and carboxyl terminal (c) which gives structural directionality. The side chains on amino acids create the different 20 amino acids. To be familiar with protein interactions and the concept of antibodies. Denatured proteins may recover their natural shape (eg. after denatured due to urea, remove urea and protein refolds to original conformation). Chaperone proteins: guide the folding of new proteins to a correct conformation.