BCH 4024 Chapter Notes - Chapter 33-45: Dna-Binding Domain, Lac Operon, Lac Repressor

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coppermouse260

20 Oct 2015

School

University of Florida

Department

Biochemistry and Molecular Biology

Course

BCH 4024

Professor

Brown Kevin

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Document Summary

33: trans acting factors- diffusible and act at multiple sites in the genome, ex: transcription factors and some regulatory rna, cis acting factors- tied closely to a gene. Immoveable: ex: dna sequence elements: promotors and binding sites for trans acting factors, consensus promoter- at -10: tataat at -35: ttgaca, mrna- copy of the coding strand of dna from a bacterial gene. Carries the base sequence encoding the amino acid sequence for a protein trna- carries an aa into catalytic site of ribosome. Base pairs to mrna rrna- structural components of a ribosome: rna transcript is the dna coding strand with u instead of t, operon- a cluster of genes regulated together. One promoter and terminator yields a polycistronic mrna: prokaryotic promoter- cis acting element in the genome where rna polymerase binds to initiate transcription, bacteria: -35 region, -10 region (spacer in between). Sigma- recognizes promoter (sigma 70 in e. coli) Hexomeric protein that separates rna from dna template.

Related Questions

Please help on these. Thank you!

Question 1

Which of the following is NOT a channel in the RNA polymeraseenzyme?

		RNA exit channel
		DNA entry channel
		DNA exit channel
		Nucleotide exit channel

Question 2

A promoter region upstream of a bacterial gene typically has thesequence TTGACA in the -35 position and TATAAT in the -10 position.If there was a 5 bp insertion at the -40 position, such thateverything downstream was shifted (ie: the "-35 element" is now atthe -30 from the transcription start site), which of the followingwill occur?

		Sigma protein will not bind and so no transcription willoccur.
		RNA polymerase will not bind and so no transcription willoccur.
		Sigma protein will bind to its target sequences and sotranscription will start 5bp downstream from the originaltranscription start site.
		Sigma protein will bind as usual and transcription will startappropriately at the transcription start site.

Question 3

A person has a mutation that prevents TFIIH from being produced.Which of the following outcomes will occur?

		No transcription because TFIID will not be able to bind.
		No transcription because the DNA strand will not be opened.
		Transcription will occur, but it will not be in the correctposition.
		Transcription will occur as usual.

Question 4

There are 4 rRNAs produced to make both subunits of theribosome. How many transcripts are required to produce these 4rRNAs?

		1
		2
		3
		4

Question 5

True or False: the 5' 7m-Gppp cap is attached to the start codonof the mRNA.

		True.
		False, the cap is attached to the 5'-untranslated region.
		False - the cap is attached to the 3'-untranslated region.
		False, the cap is attached to the polyA tail.

Question 6

During the splicing mechanism, which of the following joins twoexons together?

		A 2'OH attacks a 5' phosphate
		A 5' phosphate attacks a 2'OH
		A 3'OH attacks a 5' phosphate
		A 5' phosphate attacks a 3'OH Question 7 After their experiment in Neurospora, Beadle and Tatum concludedthat each single genes code for single enzymes. Do you agree withtheir statement, why or why not?

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BCH 4024 Chapter Notes - Chapter 33-45: Dna-Binding Domain, Lac Operon, Lac Repressor

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