BIMM 120 Chapter Notes - Chapter 1: Bacillus Cereus, Membrane Potential, Endospore

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26 Oct 2018
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How does bt work: various strains and insecticidal proteins, bt has large diversity. Insecticidal activity due to intracellular crystal inclusions produced during sporulation: crystals comprised of icps encoded by cry and cyt genes located mainly on plasmids. Killing takes hours to days: crystals ingested by insects dissolve and the proteins are activated by proteases in the juices of the midgut. Forms pores, leading to loss of transmembrane potential, cell lysis, leakage of midgut contents, paralysis, and death. Insects that develop resistance usually exhibit decreased/altered receptor binding: variability and low-dosage residues on plant may contribute to emergence of resistant insect populations. In soil, stability of icps affected by microbial degradation. Drier soil allows stable icps and less microbial degradation. Current uses of bt: sprays, active ingredient: viable bacterial spores, cry and cyt icps are primary cause of insect death, spore germinates after midgut is damaged. Bt exposed on plant surfaces highly sensitive to degradation by uv light and removal by water.

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