LIFESCI 23L Chapter Notes - Chapter E: Fasta Format, Reverse Transcriptase, Thymidine Triphosphate
Document Summary
Mitchondrial dna from mother only, is haploid --> (haplotypes) Use polymerase chain reaction pcr to amplify dna for analysis. Cheek cells" dna region = control region (hypervariable segment i) not protein coding region so it can have more mutations. 600 bp hypervariable region in mtdna (16k bp total) Data collected will only identify inclusion in broad haplogroup, reflect shared migration patterns dating back, cannot identify you. Chelex binds mg2+ so enzymes like dnase will not degrade dna. Primers bracket region of interest, dna pol adds nucs. Use heat resistant bacteria dna pol for this lab so it is denaturation resistant. Raise then lower heat to repeat synthesis (35x) Melt, anneal, extend --> double dna each cycle. Can detect mutation using only small amount from skin sample, use to detect. C/g make up 50 - 60% of total bp. Cg in 3" end bc binds more tightly. Forward/left sequence = reference strand sequence, binds to complement strand.
