BIOL 110 Chapter Notes - Chapter 19: Semiconservative Replication, Sister Chromatids, Nucleic Acid Double Helix
Tutorial 19
Thursday, October 20, 2016
11:32 AM
DNA Replication
• DNA replication
o S-phase
o Chromosome comprised of two identical sister chromatids
o Double helix unwinds and nitrogenous bases are added to each strand of the parent
molecule, two identical copies of the parent strand
o Semiconservative replication - each newly formed DNA has one stand conserved from the
parent molecule and one synthesized strand
o Origins of replication
o Enzymes
• Helicase - unwinds the DNA double helix
• Single strand binding proteins - keep the strands separate
• Primases - initiate replication
• DNA polymerase - adds nucleotides to the unwound parent molecule
o The strands have opposite chemical polarities
• H-bonding between bases is caused by the opposite polarities
• Antiparallel - one is the 3'-to-5' strand and the other the 5'-to-3' strand
o DNA polymerase
• Limitation; it can only add nucleotides to the 3 end of the newly synthesized strand of
DNA
• Nucleotide addition is smooth and continous on the leading strand
• It is discontinous on the lagging strand
▪ DNA polymerase can only work by starting at from the replication fork (where
the DNA is unwinding) and progressing outward
• Lagging strand
▪ Primase has the ability to synthesize a short primer made of RNA nucleotides
▪ Okazaki fragments
• Short stretches of DNA
• Ligase seals these fragments to make them continuous
• Errors During DNA replication result in mutations
o Mistakes occur about once in every 10000 base pairs
o Observed error rate is very low
o Mismatch repair occurs when DNA polymerase and other proofreading enzymes remove
incorrectly paired nucleotides
o Excision repair involves the removal of damaged nucleotides from a DNA molecule
o Mutation is a permanent change in an organism's DNA
• If it occurs in a reproductive cell, the mutation can be passed on to future generations
and possible be established in a population
• Harmful - sickle cell, CF
• Beneficial - antibiotic resistance
• Neutral - DNA sequence changes but it has no effect on the phenotype
• There are enzymes that defend against mutations
▪ XP (Xeroderma pigmentosum)
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