PLSC 363 Chapter Notes - Chapter 4: Intermediate Filament, Peroxisome, Endomembrane System
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Magnification - ratio of an object"s image size to its real size resolution - measure of the clarity of the image (min distance two points can be separated and still be distinguished) Contrast - difference in brightness between the light and dark areas of image. Organelles - the membrane-enclosed structures within eukaryotic cells. Electron microscope - focuses beam of electrons through a specimen or onto its surface shorter wavelengths than light transmission electron microscope - used to study internal structure of cells. Scanning electron microscope - used for detail study of the topography of a specimen. Electron microscopy kills the cells fluorescent markers for light microscope make it easier to see. Broken up cells placed in tube. spun. New tube spun again. repeat. largest settle on bottom pellet liquid above pellet poured in new tube. 4. 2 eukaryotic cells have internal membranes that compartmentalize their function. Cytosol - semifluid, jellylike substance in cells.