KIN 2577 Chapter : 4 School Wellness And PA Programs 3

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azuremammoth471

15 Mar 2019

School

LSU

Department

Kinesiology: Professional Courses/Basic Activity Courses

Course

KIN 2577

Professor

All

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Document Summary

Correct answer: 3. 0: volumetric, serological, mohr, mechanical, what is the size of the pipet shown above, 1 ml, 2 ml, 5 ml, 10 ml. Just type in the volume, not the units (e. g. 0. 50, not. Your response: 6. 7: you are asked to pipet 2. 6ml into a reaction mixture. There are four pipets on the lab bench (a, b, c, Which one do you use: p100 micropipet, 1. 0ml pipet, why did you choose this pipet, 5. 0ml pipet, 10. 0ml pipet. Your response: its the smallest pipet just big enough to hold 2. 6ml. Correct answer: greatest accuracy: arrange the following steps in a possible correct order for calibration of the spectrophotometer. 1 is first and 6 is last: switch mode to absorbance. Correct answer: a:6, b:4, c:5, d:1, e:3, f:2. Your response: a:5, b:4, c:6, d:2, e:3, f:1: 1, 2, 3, 4. Correct answer: b: as the concentration of a colored solution _______, the __________ value ___________, increases; absorbance; increases, decreases; absorbance; increases.

Related Questions

I tried posting this question before, but I didn't get a greatanswer. ÃÆPlease this is for the ANION test lab NOT the CATIONtest. Thank you!

The lab is for CHEM II and it is taken out of a lab paq. Below arethe materials used.

Classify your ions according to the following three groups.

Several of the cations belong to more

than one group.

Group 1: Anions that WILL NOT PRECIPITATE in the presence of

silver nitrate.

Group 2: Anions that WILL PRECIPITATE in the presence of silver

nitrate and the resulting

precipitates WILL DISSOLVE upon acidification with nitric

acid.

Group 3: Anions that WILL PRECIPITATE in the presence of silver

nitrate and the resulting

precipitates WILL NOT DISSOLVE upon acidification with nitric

acid.

A. Write a net ionic equation for any reaction that produces a

precipitate.

B. Identify the anions that produce gas upon addition of

HCl.

C. Identify the anions that do not precipitate with silver

nitrate.

D. Identify the anions that react with silver nitrate to form

precipitates that dissolve when

acidified with nitric acid.

E. Identify the anions that react with silver nitrate to form

precipitates that do not dissolve when

acidified with nitric acid.

F. What simple test (other than a specific confirmation test)

would distinguish between CO3 2-

and NO3 - ?

Here are the materials we used

1 Ammonium Molybdate, 0.2 M - 2 mL in Pipet

1 Aqueous Ammonia, 6 M, NH4OH - 4 mL in Pipet

1 Barium Chloride, 0.3 M - 2 mL in Pipet

1 Hydrochloric Acid, 6 M - 8 mL in Dropper Bottle

1 Nitric Acid, 6 M - 2.5 mL in Pipet

1 Organic Reagent - 1.5 mL in Vial

2 Pipet, Empty Short Stem

1 Silver Nitrate, 0.1 N - 6 mL in White Dropper

Bottle

1 Sodium Bromide, 0.1 M - 2.5 mL in Pipet

1 Sodium Carbonate, 0.25 M - 2.5 mL in Pipet

1 Sodium Chloride, 0.1 M - 2.5 mL in Pipet

1 Sodium Iodide, 0.1 M - 2.5 mL in Pipet

1 Sodium Phosphate, 0.1 M - 2.5 mL in Pipet

1 Sodium Sulfate, 0.1 M - 2.5 mL in Pipet

1 Sodium Sulfide, 0.1 M - 2.5 mL in Pipet

1 Unknown - 5 mL in Pipet

Prepare Mg standards: Dilute 100-ppm Mg standard stock solution (you made previously) to the following concentrations in the previously cleaned test tubes. Use volumetric pipets to transfer the liquids. Again this is your standard solutions whose concentration accuracy is critical to your results. Treat the pipet used to transfer the standard solution with all the proper cleaning/coating steps. Read the markings on the pipet carefully during transfers.

[Mg] made Volume of 100-ppm Mg Volume of d.i. water

(use 5-mL grd. Pipet) (use 10-mL grd. Pipet)

50 ppm 5.00 mL 5.00 mL

25 ppm 2.50 mL 7.50 mL

12.5 ppm 1.25 mL 8.75 mL

Mix the solution in each test tube well by capping and inverting the text tubes.

At this point, place another seven (7) clean test tubes in a test tube rack. Label them 1 to 7.

2. In test tube 1 pipet 0.5-mL d.i. water. In Test Tubes 2-4 pipet 0.50-mL of three Mg standards you just made, one solution to one test tube. In Test Tubes 5-7 pipet 0.5-mL of the bovine serum sample.

3. Add 2.5-mL of TCA to each test tube prepared in Step 2, cap and mix well, and let stand for 5 minutes.

4. Centrifuge all 7 test tubes for 5 minutes. Make sure that you place the test tubes in the centrifuge in a symmetrical pattern so that the spinning will not be off balance. The test tubes will break if off-balance spinning occurs.

5. Use a pipet to transfer 2.00-mL of each solution or the supernatant solution from Step 4 into another set of 7 clean test tubes. To each transferred solution, add 2.00-mL of the TY working solution you made today and 1.00-mL (with a pipet) of 2.5M NaOH solution. Cap, mix well, and let stand for 5 minutes.

6. Bring these seven solutions to a Spectronics 20D spectrophotometer. Set the monochromator to 540nm. Zero the Transmittance with nothing in the cuvet chamber and then zero the Absorbance with water in the cuvet chamber. Measure the absorbance of each of the seven solutions, standards first from the least concentrated to the most concentrated, and then the serum samples.

Based on the procedures delineated in Steps (1)-(6), draw a "flow chart" to follow the path of your analyte Mg. Establish proportional relationships between (1) ppm Mg in serum calculated from the calibration curve with that in the original serum sample; (2) ppm Mg from the calibration curve with that in the curvet. (E.g., if you calculated 10-ppm Mg off the graph, what is it in the serum sample? In the cuvet?)

KIN 2577 Chapter : 4 School Wellness And PA Programs 3

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