BIOL 4141 Chapter : Lab Manual For Mammals
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Using the table below, explain the purpose of each of the steps of the Experimental Flow Diagram (Figure 1 from your lab manual) for answering the two research questions guiding this laboratory investigation:
Is the bacterial contamination at the three farms due to the same plasmid or different plasmids?
What is the frequency of antibiotic resistant bacteria in the farm cultures?
Box | Why are you doing this step? | How is this step relevant to research question(s) 1 and/or 2? |
Week 1: Experiment Part A | ||
Week 2: Experiment Part B | ||
Week 2: Experiment Part C | ||
Week 2: Experiment Part D | ||
Week 3: Calculate frequency of antibiotic resistance at farms | ||
Week 3: Use size markers on gel to determine size of resistance genes | ||
Week 3: Compare size to known size of resistance genes to identify which gene was present in each farm |