BIOL 4087 Chapter : Biochem
Document Summary
Study of the chemistry of organisms, their life processes, and their environment. A+b c + d: write 5 things about reaction you would want to know to understand it, foundations to biochemistry: cellular, chemical, physical, genetic, At 8. 95 charge goes to 0, 10. 53 gets a charge. Answer if midpoint between 8. 95 and 10. 53. answer is close to 10. Know structures of amino acids: cysteine: not exactly polar. It has very important role in protein structure. The bond stablization around r group doesn"t allow that to occur: basic amino groups have protonated nitrogen, the isoelectric point is the point of electrical neutrality. What active site: crude extracts: differential centrifugation, salting out: differential solubility, salt: ammonium sulfate occupies the water so the protein precipitates, protein is collected and the salt removed by dialysis. Collumn chromatography: diminishes dilution effect, can take advantage of lots of characteristics of proteins.
