10. 3 using molecular probes to find and analyze a specific clone of interest recombinant dna molecule containing the gene of interest. A library has thousands of cloned dna fragments that must be screened to find the. This is accomplished using specific probes that will find and mark only the. 1. those that recognize a specific nucleic acid sequence. 2. those that recognize a specific protein collision. the double stranded hybrid is stable. Probing for dna makes use of base complementarity. Two single stranded nucleic acids will find each other in a solution by random. Probing for dna requires that all molecules be made single stranded by heating. Finding the clone of interest by using dna/rna probes: Collection of bacterial cells containing a fosmid library-transfer colonies to absorbent membrane-incubate membrane with radioactive probe-autodiagraph to locate desired clone, spot will show the desired clone-amplify desired gene. Where does dna to make a probe come from? of the gene of interest.