MCB 3020 Chapter Notes - Chapter 12: Deoxyribonucleotide, Lyme Disease, Human Papillomavirus Infection
Document Summary
Each frag(cid:373)e(cid:374)t"s (cid:373)igratio(cid:374) rate is i(cid:374)versely proportional to the log of its molecular weight. Southern blotting technique: edwin m. southern (1975) developed 3 step process for use in detecting specific. Separate dna molecules transfer separated dna molecules. First recombinant molecule: 1982 genetically cdna fragment coding for human insulin recombined into e. coli vector, produced new source of human insulin, first commercial use for recombinant molecules. Pcr reaction: enables gene amplification the rapid synthesis of many copies of a specific dna fragment from a complex mixture of dna and other cellular components. Chain length can vary from short (2 30 nucleotides) to long (50 . Pcr reaction mix: reaction mix contains. Primers target dna thermostable dna polymerase (e. g. taq polymerase) Each of the four deoxyribonucleotide triphosphates: thermocycler is the instrument used in the reaction. Copies of the target dna are synthesized, i. e. , amplified.
