BOT 4734C Chapter Notes - Chapter 2: Open Reading Frame, Start Codon, Polyadenylation
Document Summary
Coding region (between promoter and terminator region: open reading frame (orf, begins with a start codon, start codons can be atg (dna) or aug (mrna) coding for methionine (met, ends with one of three stop codons: Do(cid:449)(cid:374)strea(cid:373) (cid:894)3"(cid:895) regulatory regio(cid:374) (cid:894)ter(cid:373)i(cid:374)ator(cid:895) at the end of a gene: 3"-u(cid:374)tra(cid:374)slated regio(cid:374) (cid:894)3" utr< after stop codo(cid:374), i(cid:374) mrna, polyadenylation signal (aataaa) Introns: various bits of noncoding segments in the gene to be removed in final. A gene may have 0 to 100s of introns. Dna recombination occurs during cell division (mitosis) The foreign dna may be incorporated into the host genome. How to introduce dna into plant cells: two commonly used methods: Biolistics (physical) particle bombardment (gene gun) Agrobacterium-mediated dna delivery into plant cells: agrobacterium: a fairly common group of soil bacteria that infects various types of dicot plants. In nature: infect mostly dicot plants; causes crown gall tumors in many species of plants. Agrobacterium cells contain ti- (tumor inducing) plasmid.
