CGS NS 202 Chapter Notes - Chapter 1: Genbank, Alternative Splicing, House Mouse
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Blasting through the kindgom of life (by sandra porter, digital world biology world) Instructions: access the (cid:862)bla t e(cid:395)ue(cid:374)(cid:272)es(cid:863) file posted o(cid:374) bla(cid:272)k(cid:271)oa(cid:396)d. in this file, you will find specific instructions for how to copy and paste these sequences into. Once you have been assigned a specific sequence, copy it into nucleotide blast to identify it, and use the information from your search to answer the questions below. Hints for each question are shown in italics. For your reference, there is an (cid:862)exa(cid:373)ple a(cid:374)swe(cid:396)s(cid:863) file also posted o(cid:374) bla(cid:272)k(cid:271)oa(cid:396)d that uses the (cid:862)exa(cid:373)ple(cid:863) se(cid:395)ue(cid:374)(cid:272)e i(cid:374) the (cid:862)bla t. This sequence is called the query sequence because you used it to ask a question (or query) of the database. Hint: refer to the slide in the blast tutorial that discusses the e value. Mus musculus mrna for alpha-amylase, salivary splice variant. The data that supports this conclusion comes from the tutorial on blast. com and comparing it to my own sequence.