BI110 Chapter Notes - Chapter 8: Tata Box, Dna Replication, Thymidine
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DNA Structure and Function LabReport
- DNA Structure
- Which two scientists are credited with discovering DNA?
- Name the nitrogen bases that are purines.
- Which nitrogen base pairs with thymine?
- List the three components of a nucleotide.
- DNA Replication
- What is the purpose of DNA replication?
- How many times does replication occur in the life of acell?
- In the Lab, Exercise 2, the original strand on the left had thebases shown below. Input the new bases that correctly pair with theoriginal strand.
Original | New |
C | |
A | |
G | |
T |
- RNA Structure
- Describe the structure and function of RNA.
- Refer to Exercise 3 and record the bases of the RNA strandproduced from the replicated DNA strand.
DNA | RNA |
C | |
A | |
G | |
T |
- Record the differences between DNA and RNA in the tablebelow.
DNA | RNA | |
Sugars | ||
Bases | ||
Strands |
- RNA Synthesis
- The process of assembling RNA is called _________.
- How is replication different from transcription?
- Refer to Exercise 4. Write the letters for the base sequence ofmRNA in the spaces below DNA. Note that the order is reversed;start with the 3â end of the DNA strand and the 5â end of the mRNAstrand. Transcription is DNA to mRNA. Note RNA contains Uracilinstead of Thymine; There is no thymine in RNA.
DNA | 3â | C | G | T | C | G | T | C | C | A | A | T | T | 5â |
mRNA | 5â | 3â |
- Protein Synthesis
- What type of RNA provides amino acids to build polypeptidechains?
- If a mRNA strand has the bases 5â CUC 3â, what amino acid willbe translated? Refer to the printable chart in Exercise 5.
- Where in the cell does translation occur?
- Genes
- What could be the problem if there is a change in the basesequence of a gene as it is passed down to the offspring?
- Give an example of a disorder that results from changes in theamino acid sequence.
- What causes sickle cell anemia?
- Gene Cloning
- What is the function of a plasmid?
- Print the document from Lab, Exercise 6. Complete the activityalong with the video demonstration. Sign, date, and take an imageof your final product and include with this lab report.
1. | In addition to identifying the genetic material, the experiments of Avery, MacLeod, and McCarty with different strains of Streptococcus pneumoniae demonstrated that | ||||||||||
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2. | In order to show that DNA in cell extracts is responsible for genetic transformation in Streptococcus pneumoniae, important corroborating evidence should indicate that _______ also destroy transforming activity. | ||||||||||
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3. | Based on what you have learned about the experiments conducted by Griffith and Avery and colleagues with bacteria, which of the following would result in transformation of living R cells? | ||||||||||
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4. | A-T base pairs in a DNA double helix | ||||||||||
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5. | If 23 percent of the bases in a sample of double-stranded DNA are adenine, what percentage of the bases are uracil? | ||||||||||
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6. | The uniform diameter of the DNA structure provides evidence for | ||||||||||
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7. | If a sequence of one strand of DNA is 5â²-TGACTATC-3â², what is the complementary strand? | ||||||||||
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8. | What structural aspect of the DNA facilitates dissociation of the two DNA strands for replication? | ||||||||||
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9. | If the MeselsonâStahl density gradient experiment had resulted in two bands of DNA molecules after only one round of replication, one containing only 15N and the second only 14N, this result would have indicated that replication was | ||||||||||
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10. | The nucleoside analogue acyclovir, which is used to treat herpes simplex virus (HSV) infections, lacks a 3â² hydroxyl group (âOH). Predict what will happen if the host cell DNA polymerase incorporates a molecule of acyclovir into an elongating strand of HSV DNA. | ||||||||||
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11. | Which of the following does not demonstrate the stability of the DNA double helix? | ||||||||||
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12. | What effect would a primase inhibitor have on DNA replication? | ||||||||||
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13. | To replicate their DNA in a timely manner, most eukaryotic chromosomes | ||||||||||
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14. | Which statement about DNA replication is false? | ||||||||||
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15. | In many eukaryotes, there are repetitive sequences called telomeres at the ends of chromosomes. After successive rounds of DNA replication, the _______ strand becomes shorter. In some cells, an enzyme called _______ repairs the shortened strand. | ||||||||||
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16. | A researcher studies normal human fibroblast cells. They can be maintained in culture but die off after about 30 cell generations. Unexpectedly, a colony of cells continues to survive and divide past 30 generations. Which scenario is most likely true for these cells? | ||||||||||
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17. | If DNA polymerase III introduces an incorrect nucleotide, what is the first corrective action made by the DNA repair system? | ||||||||||
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18. | Choose the correct order of the following four events in the excision repair of DNA: (1) Base-paired DNA is made complementary to the template. (2) Damaged bases are recognized. (3) DNA ligase seals the new strand to existing DNA. (4) Part of a single strand is excised. | ||||||||||
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19. | Six complete cycles of PCR should result in a _______-fold increase in the amount of DNA. | ||||||||||
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20. | When double-stranded DNA is heated to temperatures above 90°C, it denatures. Denaturation is a process that | ||||||||||
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