Biochemistry 2280A Chapter Notes - Chapter 1: Transmission Electron Microscopy, Scanning Electron Microscope, X-Ray Crystallography
18 Jul 2016
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Biochemistry 2280 topic 1 fundamental concepts of biochemistry. 1 cells: the fundamental units of life. Light microscopes use visible light to illuminate specimen, allowed scientists to see first cells. In 1930s, electron microscopes used breams of electrons to capture finer details: individual large molecules visible. Invention of light microscope led to discovery of cells. Microscope used to study cell cycle and division: cell theory, that cells are generated from existing cells, confirmed in 1860s by. Light microscopes allow examination of cells and some of their. Cells separated by extracellular matrix (protein fibers in polysaccharide gel) Each cell 5-20 m in diameter in plant/animal tissue. Organelles can be distinguished using dye/stain or optical techniques (components have slightly different refractive indices) Fluorescence microscopes have resolution of approximately 20 nm size of ribosome. Fine structure of cells is revealed by electron microscopy. Sliced sections of cells have to be thinner than in light microscopy.
