BCH311H1 Chapter 3: BCH311 Andreopoulos.pdf
10 Apr 2013
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Document Summary
Nucleophilic attack by 3" oxygen to alpha phosphate: coli rnap. 2 metal ions stabilize with 3 asp residues. Strong promoters frequent transcription, more substitutions less transcription. Two step process involving trigger loop and bridge helix. Palindromic and complementary gc region followed by 4+ u. Binds to c rich rut (rho utilization) site. Actinomycin- intercalates dsdna (will affect dna but goo in small concentrations) Rnap ii has c-term domain with repeats of: ysptsps. Amanitin from deadly mushroom that can inhibit elongation phase. Genes always expressed have in -40 to -150. Binds rnapii rap30 and rap74 subunits cdt phosphorlyation. Glucose regulates as well camp regulatory protein (crp) binds camp from pyruvate generation. Enhancement of eukaryotic transcription- tata box binding protein (tbp) Tandum repeats of 4 zinc-cysteine by alpha helix. Alpha helix structures with leucines at 7 residues apart. Estrogen receptor binds as dimer has 3 domains. Tamoxifen binds helix 12 as well, but does not cause conformational change.
