KIN217 Chapter Notes - Chapter 5: Antiserum, Antigen, Multiple Myeloma
15 Oct 2016
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In order to understand how proteins work in the cell, we must examine them in vitro must isolate/purify them. 5. 1 the proteome is the functional representation of the genome. Proteome types, functions, and interactions of proteins that yield a functional unit. Tells us which proteins are functionally present. Genome tell us the products that could be present (fixed characteristic) Not a fixed characteristic varies w/ cell type, developmental stage, envtal conditions. Proteins do not exist in isolation interact w/ one another to form complexes w/ specific functional properties. An understanding of the proteome is acquired by isolating, characterizing, and cataloguing proteins. 5. 2 the purification of a protein is the first step in understanding its function. To understand how a protein functions, we must isolate the protein of interest from the thousands of other proteins in the cell. Protein sample may be a small fraction of the starting material. We will examine the purification of 2 proteins.
