BIOL342 Chapter Notes - Chapter 6: Cyclic Adenosine Monophosphate, Escherichia Coli, Fusion Protein
7 Sep 2013
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Remember that the main purpose of gene cloning is to get the expression of the cloned gene in a host organism. However, sometimes inserting a gene into a cloning vector doesn"t always result in the gene being expressed. For many biotech purposes we require high amounts of protein production. There are many ways to manipulate gene expression: Via promoter and terminator sequences (transcriptional control) There is no single strategy to get the maximal gene expression of a cloned gene. The level of gene expression also often relies on the host organism. A main requirement for gene expression is to have a strong and regulatable promoter. A strong promoter will have a high affinity for rnapol. Being able to regulate a promoter will allow for control of transcription. It might seem like it"s a good idea to express a cloned gene through a continuously activated and strong promoter.
