BIOL240 Chapter Notes -Lambda Phage, Holliday Junction, Sos Response
18 Mar 2013
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Document Summary
Part a: an introduction to the terminology of molecular biology. History: coli and salmonella developed in first genetic system. Mutation: change that occurs within gene to make another allele of gene or disrupt function of gene. Mutation gain/loss of function, change of function at dna level. Genotype: description of all alleles within organism. Phenotype: observable properties of strain containing mutation. Wild-type: strain found in nature original isolate of strain that all mutants are derived from. Part b: the phenotypes of mutations selection vs. Bacteria plated out on growth medium under certain conditions that will allow phenotype of interest to grow. Power of genetics ability to use selection vs. screening. Screenable phenotype cannot be selected but can be differentiated. Error occurring during replication leads to evolutionary change. Culture of wild-bacteria subjected to mutagenic conditions to increase mutation rate. Culture diluted and placed on complex medium tor single colonies on master plate. Auxotroph: organism that developed nutritional requirement through mutation.
