MBG 2040 Chapter 11: Chapter 11 notes

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coralraccoon960

9 Mar 2016

School

University of Guelph

Department

Molecular Biology and Genetics

Course

MBG 2040

Professor

Nicole Robbins

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Document Summary

Dna to protein involves 2 steps: transcription: the transfer of genetic information from dna to rna. One strand of dna is used as a template to produce complementary strand of rna (the gene transcript: translation: the transfer of information from rna to protein. The sequence of nucleotides in the rna transcript is converted to the sequence of amino acids in the polypeptide gene product. Takes place on ribosomes, composed of 3-5 rna molecules and proteins. Primary transcript: the product of transcription, the same as mrna in prokaryotes. Pre-mrna: in eukaryotes, the precursor to mrna; the primary transcript must undergo excision introns and medication of both ends before translation. Bind to rna making it double stranded, which blocks translation, control protein production. *note: only mrnas encode protein, the rest are structural. The process of gene expression: overview in eukaryotes. Similar to dna synthesis but the precursors are ribonucleoside triphosphates (rtps) rather than deoxynibonucleoside triphosphates.

Related Questions

When we use DNA to code for a protein, we call the process Transcription and Translation. Below are descriptions of processes that happen when we use DNA to code for a protein. Match the description of each process with the correct term - either Transcription or Translation.

1.A ribosome reads the mRNA and builds a protein from amino acids.
2.A copy is made of a gene in RNA
3.The primary transcript is modified: introns are removed and the transcript gets a cap and a tail
4.tRNA carries amino acids to a ribosome
5.The DNA unwinds, exposing the gene to be copied.

Fill in the blank. Elongation during translation does NOT involve ____________.

Question 16 options:

	the translation of codons according to the genetic code
	the formation of bonds catalyzed by the ribosome
	complementary base pairing between RNA molecules
	amino acids being linked together in a polypeptide
	reading the DNA template 3' to 5'

For a given gene, what establishes the reading frame for translation?

Question 17 options:

	the location of the enhancer relative to the gene
	the first three nucleotides at the 5' end of the mRNA
	the first three nucleotides at the 3' end of the mRNA
	the start codon in the mRNA
	the location of the promoter relative to the gene

Which of the following is the LEAST likely direct consequence of a substitution mutation?

Question 18 options:

	changing the length of a protein coded for by a gene
	changing one amino acid in a protein
	creating a stop codon
	eliminating a start codon
	changing the length of the DNA molecule containing a gene

Suppose that the pre-mRNA transcript from a eukaryotic gene is 30,000 nucleotides long, and the gene codes for a sequence of 300 amino acids. What is the best explanation for the relationship between these numbers?

Question 19 options:

	only the first 900 nucleotides of the pre-mRNA transcript are translated
	it takes 100 nucleotides to specify a single amino acid
	300 of the nucleotides in the transcript are important, and the rest are "junk"
	only the last 900 nucleotides of the pre-mRNA transcript are translated
	large portions of pre-mRNA transcripts are cut out during RNA processing

Suppose an individual is born into a population with a novel mutation. Is the new mutation an evolutionary change, and why?

Question 20 options:

	no, because it is not a big enough change to count
	yes, because new mutations are always adaptive
	yes, because the appearance of a new genetic variant is a genetic change in a population
	no, because not enough individuals have the mutation for it to matter
	no, because most mutations are not adaptive

1. _________________________ is the name of the process that produces proteins using RNA molecules.

a.Transcription

b Translation

c. Replication

d. Mutagenesis

2. The peptide bonding site on the ribosome:

a. A site

b. P site

c. E site

d. D site

3. The name given to the bond formed between two amino acids.

a. Hydrogen bond

b. Double bond

c. Peptide bond

d. Negative bond

4. __________________________ is the movement of the mRNA molecule on the ribosome to open up the A-site for the new incoming amino acid.

a. Transformation

b. Mutation

c. Translocation

d. Initiation

5. What does the term semi-conservative mean.

a. Replication of RNA is semi-conservative, each new strand consists of one old strand of RNA and one new strand of RNA.

b. Replication of DNA is semi-conservative, each new strand consists of one old strand of DNA and one new strand of DNA.

c. Replication of DNA is semi-conservative, each new strand consists of new strands of nucleotides.

d. Transcription of RNA is semi conservative.

6. This molecule functions during protein synthesis to recognize the codon and bring the proper amino acid back to the ribosome for translation.

a. mRNA

b. tRNA

c. rRNA

d. DNA polymerase

7. What is meant by the base-pairing rule.

a. Bases on a single strand of DNA are always found as pairs

b. Adenine always base pairs with guanine; cytosine with thymine

c. Adenine always base pairs with thymine; cytosine with guanine

d. Bases on a single strand of RNA are found in pair.

8. In this step of protein synthesis, amino acids are added to a growing polypeptide chain.

a. Initiation.

b. Elongation.

c. Termination.

d. Transpiration.

9. Three complementary bases found in a mRNA are called ____________________.

a. Anti-codon.

b. Triplet.

c. Doublet.

d. Codons.

10. Which statement best describes the job or function of the messenger RNA.

a. Carries the map of the gene sequence in the form of codons to the cytoplasm

for translation of the protein.

b. It transfers amino acids to the ribosome for protein synthesis.

c. It forms the ribosomes.

d. It helps replicate DNA.

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MBG 2040 Chapter 11: Chapter 11 notes

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