CHEM 271 Chapter Notes - Chapter 9: Enzyme Catalysis, Carbonic Anhydrase, Restriction Enzyme
16 Apr 2016
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Document Summary
Catalyic strategies used by four classes of enzymes: serine proteases, carbonic anhydrases, restricion endonucleases, and myosins: each class catalyses reacions that require the addiion of water to a substrate. For serine proteases, exempliied by chymotrypsin, the challenge is to promote a reacion that is almost immeasurably slow at neutral ph in the absence of a catalyst. For carbonic anhydrases, the challenge is to achieve a high absolute rate of reacion, suitable for integraion with other rapid physiological processes. For restricion endonucleases such as ecorv, the challenge is to atain a high degree of speciicity. For myosins, the challenge is to uilize the free energy associated with the hydrolysis of atp to drive other processes. A few basic catalyic principles are used by many enzymes. The binding energy is the free energy released in the formaion of a large number of weak interacions between the enzyme and the substrate.
