BIOL 367 Chapter Notes - Chapter 5: Dimethyl Sulfate, Scintillation Counter, Deoxycytidine Triphosphate
Document Summary
Chapter 5 molecular tools for studying genes and gene activity. Isolation and analysis of dna, rna and protein. Runs an electric current through the gel at neutral ph. Dna neg charged due to phosphates in the backbone so they migrate towards anode. Dna molecules experience little frictional drag from solvent and gel molecules, so they migrate rapidly. Dna is colorless so you must stain dna or make radioactive. Electrophoretic mobility (migration rate) of a dna fragment is inversely proportional to the log of its size. Agarose gel electrophoresis of very large dna molecules (orthogonal gel electrophoresis or alternating field) Can separate molecules up to several million base pairs long. Uses pulses of current, with relatively long pulses in the forward direction and shorter pulses in the opposite, or even sideways, direction. Need to treat with detergent (sodium dodecyl sulfate sds) to denature the subunits so they no longer bind to one another.
