L41 BIOL 4810 Study Guide - Midterm Guide: Rna Polymerase Ii, Rna Polymerase I, Transcription Bubble

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Transcription continues until the polymerase finds a terminator site: prokaryotes: transcripts can be polycistronic/polygenic, means can transcribe entire sequence that contains multiple different proteins, each with its own gene but connected with other genes. Think lac operon with gene z, y, and a: composition: Subunit: per rnap molecule: initiates chain, interacts with regulatory proteins and upstream promoter elements. (cid:533) subunit, initiates chain, elongates chain (cid:533)" subunit, binds to dna. Kmno4 (oxidizes bases in single-strand nucleic acids): factors bind to -35 and -10 region of dna. Binds to the -10 region: the template strand has a sharp bend when bound to rnap, the 2 mg2+ ions allow ribonucleotides to polymerize. " end of growing rna is adjacent to a catalytically needed mg2+ ion. The wall of rnap forces dna to turn. rntps enter the active site through a funnel structure and pore.

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