Biology And Biomedical Sciences BIOL 2960 Study Guide - Midterm Guide: Dna Polymerase Iii Holoenzyme, Dna Polymerase I, Dna Clamp
6 Feb 2019
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In bacteria pcra 4 subunits (a1, a2, b1, b2: a1 ntp-ase p-loop, binds/hydrolyzes atp. In eukaryotes dnab - hexamer: single-strand binding proteins (ssbp) bind to ssdna to keep it unwound after helicase, dna pol requires rna primer (to donate 3"-oh) provided by rna primase (primosome complex, no. Cancer inhibit to kill cancer, aging express to maintain chromosomes after many replications: error repair, gene mutation (sequence) vs. chromosome mutation (structure/number, sequence fidelity - 5"-3" pol = 10-5/3"-5" exonuclease ( ) = 10-7/10-8, mutagens. In-vitro dna synth of synthetic oligonucleotides: template + dntp"s + primers (ss, ~20-25 nt long) + taq. I (rrna 18s, 5. 8s, 28s) tata box-like rinr (ribosomal initiator element), 100-200 bp upstream. Ii (mrna, snrna) tata box, caat box, gc box enhancer sequences, strongly inhibited by amanitin, dpe (downstream core promoter element) Igs internal guide sequence brings in guanosine: group ii, mitochondrial genes, not completely self-splicing, but sequence is essential, uses mg2+ as cofactor, better regulation of splicing.
