BIOCHEM 501 Study Guide - Chymotrypsin, Menton, Proline

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A buffer system is composed of a weak acid/weak base pair. An alpha-helix is a secondary protein structure that forms a right handed spiral with 3. 6 residues per turn. In addition an alpha helix forms n-4 hydrogen bonds where n is the number of residues in the helix. The hydrophobic effect is almost exclusively responsible for protein stability. Protein purification: what are some techniques in protein purification that take advantage of these properties to separate proteins, charge: Ion exchange chromatography (positively charged proteins stick to negatively charged beads). Isoelectric focusing: specificity, reverse phase chromatography, hydrophobic interaction chromatography, polarity, dialysis and ultrafiltration, gel electrophoresis, gel filtration chromatography, ultracentrifugation, size: Vmax signifies the maximum rate of conversion while km is generally the dissociation of the enzyme complex into the enzyme and product, this assumes very little of the product exists, limiting any equilibria.

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