BIOL 331 Study Guide - Midterm Guide: Diffraction Grating, Attenuation Coefficient, Field Lens
Document Summary
Bio 331- exam 2 study guide: spectrophotometry, general terms associated a. i. Transmittance = light that passes through cuvette but is not absorbed: components of single beam spectrophotometer (diagram pg 45) b. i. Lamp, field lens, entrance slit, light path, objective lens, diffraction grating, exit slit, sample cuvette, filter, measuring phototube, absorbance or transmittance meter: beer-lamber law: a = ac c. i. A= absorbance c. ii. a= absorption coefficient (ml/g/cm) or molar absorption coefficient (l/mole/cm) c. ii. 1. depends on what concentration is measured in c. iii. Important to zero machine every time you change the wavelength when creating absorbance spectrum: standard curve e. i. Plot of absorbance vs concentration with regression line e. i. 1. Slope of regression line is a : bacterial growth curve, exponential growth- doubles at each generation, generation time- time between cell divisions, growth curve c. i. Lag phase- no growth, cells are preparing to replication c. ii. Cell are replication constantly and generation time = doubling time c. iii.
