BIOL 151 Study Guide - Midterm Guide: Gel Electrophoresis, Electrophoresis, Electric Current
Document Summary
Explain how molecules such as dna can be separated by gel electrophoresis. Be able to interpret dna sequencing results: combine the following, dna sample, primer, dna polymerase, normal nucleotides (a, t, g, c, dideoxynucleosides: (lack an oxygen at 3") Step 1: dna must be denatured and converted from double stranded to single stranded through the application of heat. Step 2: dna splits and a primer is added to the template dna to allow the addition of nucleotides later. Step 3: create 4 reaction mixtures are set up and dna is added to the template strand with attached primer. Step 5: add normal nucleotides which helps determine the sequence later. Step 6: modified nucleotides added (only 1 type per rxn mixture) Step 7: now it occurs because dideoxynucleosides lack an o at 3". Step 8: dna must be separated so each mixture is separated into 4 wells in gel electrophoresis and each.
