MCB 150 Study Guide - Midterm Guide: Histone H1, Chromatin, Chromosome

• School: University of Illinois
• Department: Molecular and Cell Biology
• Course: MCB 150
• Professor: Brad Mehrtens

Document Summary

Chloroplasts + mitochondria come from ancestral bacterial cells: cells had their own genomes, circular double stranded dna also found in these. Packing dna to get things out of the way and to be able to access dna. Eukaryotes: 6 billion base pairs of dna in diploid genomes (half from mom half from dad: chopped into chromosomes (segmented, all the chromosomes > 2 meters in length. Must cram it together to be 1-10 micro meters (nucleus/nucleoid region) Roger kornberg: crack open cell--> take out nucleus crack nucleus take out chromatin. Beads on a string: (cid:498)beads(cid:499)=protein, (cid:498)string(cid:499)=dna. Stretch out chromatin: (cid:498)beads on a string(cid:499) Nuclease: a family of enzymes capable of hydrolyzing phosphodiester linkages (degrading nucleic acid) Just long enough to cut the string in between the beads (not the string wrapped around the beads) linker dna. Separated spots: nucleosomes: salt preparation: string falls off the beads touching the beads. String=double stranded dna w/ 146 base pairs that were.