GENE 3200 Study Guide - Midterm Guide: Multiple Cloning Site, Genetic Screen, Dna Ligase
Document Summary
Describe each of the following terms or techniques: genetic screen, genetic screens help id traits you are looking for with mutations. Dna ligase to make these stick together: vector- different types of cloning vectors can be used to isolate dna, cloning vectors- used to carry (clone) foreign dna fragments being examined. Fragments: dna clone-copy of a dna region manipulated independently of the genome (easier to work with, can modify dna without altering other regions of genome (rna, dna, or pcr product). You want a, but you can get a, b, or c ^^ The gene and plasmid are cut with the same restriction enzyme and mixed together. Dna ligase is used to seal nicks in the sugar phosphate backbone. Dna fragments can be inserted into cloning vectors, stable pieces of dna that will replicate within a cell. A cloning vector must have an origin of replication, one or more unique restriction sites, and selectable markers.
