PCB 3063 Study Guide - Midterm Guide: Dna Gyrase, Dna Replication, Thiol
17 Dec 2018
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Dna polymerase iii is the main synthesizing enzyme in dna replication in prokaryotes. Dna polymerase i removes the rna primers and lays down dna in place of it. Rna that"s being transcribed, and the six subunits will bind like a donut around the consensus sequence. It follows along the polymerase and pulls the rna that"s been transcribed off of the main dna strand. Rho- independent needs a hairpin formation: topoisomerases: which ones need atp and which ones don"t, topoisomerase ii and gyrase need atp but the others do not. Topo i uses a substrate-type mechanism for energy: remember that cysteine (and methionine) has a sulfhydryl group, meaning it can form disulfide bonds. Tata box: the one that differs the most from any other will give the lowest, bacterial promoters have a -10 tata sequence and a -35 cg sequence, but in eukaryotes the tata sequence is at -25.
