LIFESCI 4 Study Guide - Final Guide: 2-Phosphoglyceric Acid, Galactose, Cistron
Name Section:
ID#
1
1. Consider the cross: Hfr: strS arg+ met+ gal+lac-lys- x F-: strS arg- met- gal-lac+ lys+ where
the genetic map is shown below (the < indicates the Origin of transfer in the Hfr strain) (8
points).
Circle the media below which would select for the most exconjugants.
a) Minimal media + streptomycin
b) Minimal media+ arginine
c) Minimal media + galactose
d) Minimal media+ arginine + galactose
e) Minimal media+ streptomycin + lactose
f) Minimal media + galactose + streptomycin
g) Minimal media + galactose + methionine + arginine
h) Minimal media+ streptomycin+ methionine
i) Minimal media + methionine + galactose + lysine
j) Minimal media + streptomycin + lactose + methionine + lysine + arginine
k) Minimal media + streptomycin + galactose + lactose + methionine + lysine
l) Minimal media + galactose + streptomycin + arginine + lysine + methionine
m) None of these above
2. (9 points) If three six-sided dice are rolled, what is the probability that the total number of
spots showing is less than 6? Show your work and Circle your answer.
= P[1,1,1] + P[1,1,2] + P[1,2,2] + P[1,1,3] =1/6^3 + 3*1/6^3 + 3*1/6^3 + 3*1/6^3 = 5/108 =
0.046
˂
met
gal
str
lys
arg
lac
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Name:
2
3. (6 points) If your blood type is O and your mother is O, what are the possible blood types for
your father? (Circle one)
a. A
b. B
c. AB
d. O
e. a and b
f. a, b, and c
g. a, b, and d
h. a, b, c, and d
4. (8 points) A testcross of AaBb gives 15% aaBb progeny. What percentage of the progeny
will be either aabb or AaBb? Show your work and circle your answer.
70%
5. (8 Points) A testcross of AaBb gives 10% Aabb progeny. What percentage of the progeny
will be aabb if the same plant is self-fertilized? Show your work and circle your answer.
16%
6. (8 Points) You took 0.3ml from a 107 dilution of the phage stock to infect bacteria. After
overnight culture, you found 21 plaques on the bacterial lawn. What is the concentration of this
phage stock? Show your work.
Phage concentration in pfu/ml =
21*107 pfu/ 0.3ml = 7*108 pfu/ml
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Name:
3
7. (16 points) Consider a population of finches with the following genotypes:
327 brown birds with genotype AA
546 brown birds with genotype Aa
127 gray birds with genotype aa
Use the χ2 analysis to determine whether this population is in Hardy/Weinberg Equilibrium.
A. What is your null hypothesis?
The population is in equilibrium for gene A.
B. If the hypothesis is true, what are your expected values? Circle your answer
p = freq (A) = (2x327 + 546) / 2000 = 0.6
q = freq (a) = 0.4
Expected
AA p2*1000 = 360
Aa 2pg*1000=480
aa q2*1000= 160
C. How many degrees of freedom are there in this problem? Circle your answer
Df = # alleles -1 = 2-1 =1
D. Determine the χ2 value. Show your work and circle your answer.
χ2 = Sum of (observed -expected)2/exptected
χ2 = (327-360)2/360 + (546-480)2/480 + (127-160)2/160
= 3.025 + 9.075 + 6.806 = 18.906
E. Does the data support the hypothesis? Why?
3.841<18.906
No. Reject the hypothesis that this population is in HWE.
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Document Summary
S: consider the cross: hfr: str the genetic map is shown below (the < indicates the origin of transfer in the hfr strain) (8 points). arg+ met+ gal+lac-lys- x f- : str arg- met- gal-lac+ lys+ where arg gal str lac lys met. = p[1,1,1] + p[1,1,2] + p[1,2,2] + p[1,1,3] =1/6^3 + 3*1/6^3 + 3*1/6^3 + 3*1/6^3 = 5/108 = 70: (8 points) a testcross of aabb gives 10% aabb progeny. 16: (8 points) you took 0. 3ml from a 107 dilution of the phage stock to infect bacteria. After overnight culture, you found 21 plaques on the bacterial lawn. Name: (16 points) consider a population of finches with the following genotypes: Circle your answer p = freq (a) = (2x327 + 546) / 2000 = 0. 6 q = freq (a) = 0. 4. Df = # alleles -1 = 2-1 =1: determine the 2 value. Show your work and circle your answer. p2*1000 = 360.
