CHEM 153A Study Guide - Final Guide: Citrate Synthase, Aconitase, Stereospecificity

• School: UCLA
• Department: Chemistry and Biochemistry
• Course: CHEM 153A
• Professor: Tienson

Document Summary

Acetyl-coa + 3nad+ + fad + gdp + pi => 2co2 + 3 nadh + 3h+ + fadh2 + gtp + coa- G o" = 31. 5 kj/mol (lyase or transferase?) (transfer 2cs from acetyl-coa to oxaloacetate) Cleave a thioester bond in acetyl-coa => release high e. Tf reaction in the forward direction (large negative deltago) Attach 2cs of pyruvate to coenzyme a (coa-sh) Start off with 4 cs of oxaloacetate and 2 cs (from pyruvate) on acetyl-coa (citrate condensing enzyme) Carbons that do not attach to oh group is from acetyl-coa. Whichever carbons that do not bind to oh group come from. Note: only carbons from oxaloacetate are bound to oh group from h2o. =>why this reaction is a stereospecific addition because aconitase is only able to recognize coo- group from oxaloacetate (i. e. 2 chemically different carboxylate due to the stereospecificity of compatible fg on the active site for substrate binding)