BCH 100 Study Guide - Final Guide: Dna Polymerase Iii Holoenzyme, Okazaki Fragments, Dna Gyrase
11 Dec 2016
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Lecture 21: dna, rna structure: terminology: central dogma, semiconservative, origin of replication, replication fork, melting, prepriming complex, dnaa, dnab, ssb proteins, dna gyrase, topoisomerase, supercoiling, primase, primer, dna polymerase, Pol i, pol ii, pol iii, processivity, polymerization, exonucleases, endonucleases, leading strand, lagging strand, In dna replication one strand will be old and the other will be new. Prokaryotes have one origin of replication while eukaryotes can have multiple. Uses atp hydrolysis to carry out this action. Bind to at-rich sites to prevent strands re-annealing - Binding also protects sugar-phosphate backbone from being cleaved by endonucleases. Lays down a short sequence of rna nucleotides (~10. For lagging strand: synthesizes a short rna sequence for each okazaki fragment. Short strand of rna that serves as the starting point of. Pol iii re(cid:373)o(cid:448)es pri(cid:373)er (cid:894)5"--> 3" e(cid:454)o(cid:374)u(cid:272)lease a(cid:272)ti(cid:448)it(cid:455)(cid:895) Main polymerizer; elongates the dna processivity how many dnts are added before dna pol polymerization exonucleases endonucleases leading strand lagging strand.
