PHY 206 Midterm: PHY 207 UMiami 250S11 Exam 2 KEY
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Two points each: a bacterial genome can be mapped only by analyzing dominant and recessive expression of a trait in merozygotes, what, false, true. You have allowed four different strains of hfr e. coli bacteria all derived from the same f+ strain to conjugate with f- bacteria. Analysis of the resulting donated strands of dna found in the recipient cells indicate the four hfr strains each donated genes in the following order. Remember that the chromosome, though circular, is three-dimensional. So any of these fragments can be rotated around horizontally. This makes it very easy to detect the gene sequence, by matching up the ones that occur together on each strand, and then reading outwards in either direction. For example: the fragment from strain ii has gly pur in the opposite direction of the fragment in strain i. So just flip the strand around, and you can read the gene sequence in both directions.
