BIL 250 Study Guide - Midterm Guide: Transposase, Cointegration, Restriction Enzyme
15 Nov 2016
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Cut & paste- move dna directly into new position. Copy & paste- replicates dna and integrates somewhere else. Encode rt and make dna copies of rna, new dna integrates. Contains only genes needed to mobilize & insert. I 1: i(cid:374) e. coli"s gla(cid:272)tose opero(cid:374), (cid:449)ith 4-19 copies in e. coli. Transposase = restriction enzyme: small direct repeats (same orientation) = target site duplications. Composite transposons: carry genes flanked by is elements. How: is elements supply transposase and itr recognition signals. Noncomposite transposons: carry genes without is elements at end. Tn3- has 3 genes: bla (b-lactamase), tnpa (transposase), tnpb (resolvase- recombination) Cointegration: moves and integrates transposon to both genemos (duplication) Replicative transposition (copy & paste): tn3 and other noncomposite. Studied: te in corn (zea mays), used to be mutator genes. Showed crossing over too c/c = white kernels c/- = purple (c- wild type) Reversion of c to c -> purple pigment and spot.
