CHEM 3310 Study Guide - Final Guide: Affinity Chromatography, Size-Exclusion Chromatography, Cytochrome C Oxidase Subunit Ii
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Just an overview of some of the high points from each chapter. Chapter 1: carbohydrates, glycosidic bond (ether bond) formed by dehydration reaction, starch: edible, alpha glycosidic bond, cellulose: inedible, beta glycosidic bonds (beta bad!, structure equals function! Km: km= (k -1 + k2) (units of concentration, i. e. m) (michaelis. K1 constant: when [s] = km, v0= vmax, km is substrate concentration that yields velocity. V0 = vmax [s]/([s] + km: going left, km gets smaller, going right, km gets bigger. O2 ---> fe (ii) shift out from plane to porphyrin plane: hemoglobin follows aspects of both the sequential and concerted models xi. Bohr effect: protons (low ph) and co2 promote t-state: decreasing ph increases [h+, (cid:271)i(cid:374)ds o(cid:454)(cid:455)ge(cid:374) (cid:449)o(cid:396)se, so it(cid:859)s (cid:271)ette(cid:396) at gi(cid:448)i(cid:374)g it up, high [co2]- more oxygen released, co2 acidifies environment xiii. T- more sigmoidal and r- more hyperbolic xiv. lower ph facilitates formation of salt bridge between beta and alpha subunits xv.
