VTPB 405 Study Guide - Midterm Guide: Beam Splitter, Gram Staining, Crystal Violet

• School: Texas A&M University
• Department: Veterinary Pathobiology
• Course: VTPB 405
• Professor: Mohammed Tawfik Omran

Document Summary

Staining: gram staining, primary stain: crystal violet. Stains both gram (+) and gram (-: mordant: iodine. Locks crystal violet into cell wall of gram (+) bacteria but not gram (-: decolorizer: acetone/alcohol mixture. Gram (+) will still be purple but gram (-) will be colorless: counterstain: safranin. Only stains gram (-: gram (+) = purple, gram (- ) = pink, acid fast staining, primary stain: carbolfuchsin. Stains both acid fast (+) and acid fast (-: mordant: heat and phenol inside the carbolfuchsin (for ziehl-neelsen), tergitol for kinyoun. Locks carbolfuchsin into cell wall of acid fast (+) bacteria but not acid fast (-: decolorizer: acid/alcohol mixture. Acid fast (+) will still be red but acid fast (-) will be colorless: counterstain: methylene blue. Fixes the malachite green dye into the cell: counterstain: safranin. Isolation and cultivation of fastidious microbes, is used to determine hemolytic reactions: enriched/differential media, results.