BIOL 222 Study Guide - Midterm Guide: Amanita Phalloides, Long Non-Coding Rna, Transposable Element

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Case study on death cap mushroom: amanita phalloides, a slow death by slowing rna polymerase, ultimately liver failure. Structure of rna: mostly produced as single-stranded, contains u instead of t, may function as 3-d folded structure following normal base pairing rules, a with u and. Template versus non-template (coding) strand of dna. Non-template mrna will have the same sequence as the nontemplate strand except u will replace t. Promoter indicates which of the two strands is the template, direction of transcription and the transcription start site. Orientation by positive numbers and negative numbers relative to start site: negative numbers upstream, positive numbers downstream. Bacterial rna polymerase: synthesize rna 5" to 3" from template dna, uses sigma factor to find, bind, and initiate transcription at promoter, rifamycins. Where to start, which strand to read, what direction: no primer required for rna polymerase to start, consensus sequence. Y either pyrimidine (c or t) R either purine (a or g)

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